ABSTRACT
Zucchini yellow mosaic virus [ZYMV] is the most prevalent virus in cucurbits in Saudi Arabia. Samples of cucumber [Cucumis sativus] and Pumpkin [Cucwbita moschata] that show symptoms of virus infection were collected during 2009 growing season from field-grown cucumber and pumpkin in Riyadh Saudi Arabia. Based on reverse transcription polymerase chain reaction [RT-PCR] analysis using one primer pairs, the virus was identified as strain of ZYMV. The PGR assay revealed that the two Riyadh isolates, ZYMVl [cucumber] and ZYMV2 [Pumpkin] were closely related to ZYMV. Serological and molecular data indicated that the two Saudi ZYMV isolates were biologically variable and presented a low molecular diversity. The Gen bank numbers were 6U808330.1 and 6U058058.1 for ZYMVl and ZYMV2, respectively. The nucleotides sequence of the two isolates ZYMVl and ZYMV2 presented molecular diversity that suggests that they did not share a common origin and they have adapted to their different hosts
Subject(s)
Polymerase Chain Reaction/statistics & numerical data , Comparative StudyABSTRACT
Zucchini yellow mosaic virus[ZYMV] is potyvirus with a worldwide distribution. The virus is the most prevalent in cucurbits. Samples of cucumber plants [Cucumls sativus L.] that show symptoms of virus infection were collected during the 18-May-2009 growing season from field-grown cucumber in Riyadh Saudi Arabia. Based on DAS-ELISA with a polyclonal antiserum against ZYMV and reverse transcription polymerase chain reaction [RT-PCR] analysis using two primer pairs, the potyvirus was identified as strain of ZYMV. The anti-ZYMV antibodies in dot-blot, confirming the identity of the isolated viral particles as a potyvirus immunogenically related to ZYMV. Nucleotides Sequence analysis confirms that the isolated virus belong to potyvirus and its gen bank is GU808330.1. This is the first report on ZYMV as causal virus infecting cucumber in KSA